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Representative images of IHC staining. IHC staining of TNBC specimens was performed as described in the Methods section with antibodies to IR, <t>IGF-1R,</t> pErk1/2, and FOXO3a. The images are shown in 40× magnification. Abbreviations: IGF-1R, IGR-1 receptor; IHC, immunohistochemistry; IR, insulin receptor; pErk1/2, phosphorylated Erk1/2; TNBC, triple negative breast cancer.
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Representative images of IHC staining. IHC staining of TNBC specimens was performed as described in the Methods section with antibodies to IR, <t>IGF-1R,</t> pErk1/2, and FOXO3a. The images are shown in 40× magnification. Abbreviations: IGF-1R, IGR-1 receptor; IHC, immunohistochemistry; IR, insulin receptor; pErk1/2, phosphorylated Erk1/2; TNBC, triple negative breast cancer.
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Representative images of IHC staining. IHC staining of TNBC specimens was performed as described in the Methods section with antibodies to IR, <t>IGF-1R,</t> pErk1/2, and FOXO3a. The images are shown in 40× magnification. Abbreviations: IGF-1R, IGR-1 receptor; IHC, immunohistochemistry; IR, insulin receptor; pErk1/2, phosphorylated Erk1/2; TNBC, triple negative breast cancer.
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Representative images of IHC staining. IHC staining of TNBC specimens was performed as described in the Methods section with antibodies to IR, <t>IGF-1R,</t> pErk1/2, and FOXO3a. The images are shown in 40× magnification. Abbreviations: IGF-1R, IGR-1 receptor; IHC, immunohistochemistry; IR, insulin receptor; pErk1/2, phosphorylated Erk1/2; TNBC, triple negative breast cancer.
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Representative images of IHC staining. IHC staining of TNBC specimens was performed as described in the Methods section with antibodies to IR, <t>IGF-1R,</t> pErk1/2, and FOXO3a. The images are shown in 40× magnification. Abbreviations: IGF-1R, IGR-1 receptor; IHC, immunohistochemistry; IR, insulin receptor; pErk1/2, phosphorylated Erk1/2; TNBC, triple negative breast cancer.
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Image Search Results


Representative images of IHC staining. IHC staining of TNBC specimens was performed as described in the Methods section with antibodies to IR, IGF-1R, pErk1/2, and FOXO3a. The images are shown in 40× magnification. Abbreviations: IGF-1R, IGR-1 receptor; IHC, immunohistochemistry; IR, insulin receptor; pErk1/2, phosphorylated Erk1/2; TNBC, triple negative breast cancer.

Journal: Journal of the Endocrine Society

Article Title: Insulin receptor expression and its association with hyperinsulinemia in triple negative breast cancer

doi: 10.1210/jendso/bvag041

Figure Lengend Snippet: Representative images of IHC staining. IHC staining of TNBC specimens was performed as described in the Methods section with antibodies to IR, IGF-1R, pErk1/2, and FOXO3a. The images are shown in 40× magnification. Abbreviations: IGF-1R, IGR-1 receptor; IHC, immunohistochemistry; IR, insulin receptor; pErk1/2, phosphorylated Erk1/2; TNBC, triple negative breast cancer.

Article Snippet: Primary antibodies were anti-IR Ab (ab137747, Abcam, Cambridge, MA, RRID:AB_3717506) 1:300 dilution, anti-IGF-1R Ab (#3027, Cell Signaling, Danvers, CA, RRID:AB_2122378) 1:200 dilution, anti-FOXO3a Ab (#12829, Cell Signaling, RRID:AB_2636990) 1:3200 dilution, and anti-pErk1/2 (Thr202/Tyr204) Ab (#4370, Cell Signaling, RRID:AB_2315112) 1:400 dilution.

Techniques: Immunohistochemistry

Representative images of protein localization for IR, IGF1R, FOXO3a, and pErk1/2. IHC staining of TNBC specimens was performed and evaluated for localization of proteins. The images are shown in 40× magnification. Abbreviations: IGF-1R, IGR-1 receptor; IHC, immunohistochemistry; IR, insulin receptor; pErk1/2, phosphorylated Erk1/2; TNBC, triple negative breast cancer.

Journal: Journal of the Endocrine Society

Article Title: Insulin receptor expression and its association with hyperinsulinemia in triple negative breast cancer

doi: 10.1210/jendso/bvag041

Figure Lengend Snippet: Representative images of protein localization for IR, IGF1R, FOXO3a, and pErk1/2. IHC staining of TNBC specimens was performed and evaluated for localization of proteins. The images are shown in 40× magnification. Abbreviations: IGF-1R, IGR-1 receptor; IHC, immunohistochemistry; IR, insulin receptor; pErk1/2, phosphorylated Erk1/2; TNBC, triple negative breast cancer.

Article Snippet: Primary antibodies were anti-IR Ab (ab137747, Abcam, Cambridge, MA, RRID:AB_3717506) 1:300 dilution, anti-IGF-1R Ab (#3027, Cell Signaling, Danvers, CA, RRID:AB_2122378) 1:200 dilution, anti-FOXO3a Ab (#12829, Cell Signaling, RRID:AB_2636990) 1:3200 dilution, and anti-pErk1/2 (Thr202/Tyr204) Ab (#4370, Cell Signaling, RRID:AB_2315112) 1:400 dilution.

Techniques: Immunohistochemistry

Overall survival for women with breast cancer based on IR and IGF-1R protein expression in KMPlot.com . KMPlot.com was used to generate overall survival Kaplan-Meier plots. The dataset used was not from our cohort but from Tang and colleagues ; n = 65 patients had IR (A) and IGF1R (B) protein expression available. The patients were split using the “auto select best cutoff” percentile option in KMPlot. No other restrictions were added to the analysis. The plots show hazard ratios with 95% confidence intervals in parentheses . Abbreviations: IGF-1R, IGR-1 receptor; IR, insulin receptor.

Journal: Journal of the Endocrine Society

Article Title: Insulin receptor expression and its association with hyperinsulinemia in triple negative breast cancer

doi: 10.1210/jendso/bvag041

Figure Lengend Snippet: Overall survival for women with breast cancer based on IR and IGF-1R protein expression in KMPlot.com . KMPlot.com was used to generate overall survival Kaplan-Meier plots. The dataset used was not from our cohort but from Tang and colleagues ; n = 65 patients had IR (A) and IGF1R (B) protein expression available. The patients were split using the “auto select best cutoff” percentile option in KMPlot. No other restrictions were added to the analysis. The plots show hazard ratios with 95% confidence intervals in parentheses . Abbreviations: IGF-1R, IGR-1 receptor; IR, insulin receptor.

Article Snippet: Primary antibodies were anti-IR Ab (ab137747, Abcam, Cambridge, MA, RRID:AB_3717506) 1:300 dilution, anti-IGF-1R Ab (#3027, Cell Signaling, Danvers, CA, RRID:AB_2122378) 1:200 dilution, anti-FOXO3a Ab (#12829, Cell Signaling, RRID:AB_2636990) 1:3200 dilution, and anti-pErk1/2 (Thr202/Tyr204) Ab (#4370, Cell Signaling, RRID:AB_2315112) 1:400 dilution.

Techniques: Expressing

Activation of the NF-κb pathway by MTEX in THP-1 recipient cells. In ( A ), NF-κb phosphorylation, p38 phosphorylation and a decrease in expression levels of TLR2 in THP-1 cells co-incubated with MTEX. In ( B ), Expression levels of p-NF-κb and p-p38 are decreased in the presence of anti-TLR2 mAb ( right ).

Journal: Cancers

Article Title: Crosstalk of Tumor-Derived Extracellular Vesicles with Immune Recipient Cells and Cancer Metastasis

doi: 10.3390/cancers18020196

Figure Lengend Snippet: Activation of the NF-κb pathway by MTEX in THP-1 recipient cells. In ( A ), NF-κb phosphorylation, p38 phosphorylation and a decrease in expression levels of TLR2 in THP-1 cells co-incubated with MTEX. In ( B ), Expression levels of p-NF-κb and p-p38 are decreased in the presence of anti-TLR2 mAb ( right ).

Article Snippet: In selected experiments, anti-TLR2 Ab (0.5 μg/mL; Cell Signaling, Boston, MA, USA 12276) or Bafilomycin A1 (BafA1, 10 nM, Sigma, 131793) were added to wells containing THP-1 cells and EVs.

Techniques: Activation Assay, Phospho-proteomics, Expressing, Incubation